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Myoridge

Myoridge is a Kyoto University spin-out company having licenses of commercialization of iPS-derived cardiomyocytes under chemically-defined and protein-free conditions on 3D platform. The CarmyA production platform provides highly purified and functional cardiomyocytes guaranteeing narrower lot-to-lot variation.  

  • Protein-free Condition
  • Chemically-defined
  • 3D Culturing Conditions
  • 90 to 98 % purity (as cTnT + CMs)
  • Excellent Drug Responding Properties 

 

News & topics

2018.06.01. Biospire starts to support Myoridge for their international business development.

Information

Category

iPS-derived cardiomyocyte, Cardiac sheet, Cardiac fibre, CRO service for drug responses

 

Business model

  • Commercialisation (distribution) of iPS-derived cardiomyocytes (cryo-preserved format)
  • CRO service for drug responses
  • Custom disease model development

 

Relationship to Biospire

Global business development supports. 

 


Location

 

46-29 Yoshidashioadachi-cho, Sakyo-ku

Kyoto 606-8501 Japan

Tel: +81 (0)75-746-7804

Please contact;

Email: info@biospire.jp

Telephone: +81(0)50-5534-0777 (Biospire Japan)


Product & Services

Function of Myoridge Cardiomyocytes – “CarmyA”

Myoridge delivers high function and quality controlled iPS-derived cardiomyocytes to the global drug discovery market.”

1. Sarcomere Structures

 

CarmyA are produced in 3D and chemically-defined culturing conditions, which induces further maturation as cardiac cells with having better sarcomere structures showing more patterned Z-bands compared with conventional 2D culturing methods.

Fig.1. Electron microscopy images of typical CarmyA (above) and Immunofluorescent staining images of CarmyA (bottom-left) and conventional 2D cultured iPS-derived cardiomyocytes (bottom-right). 

Fig.2. Percentage of patterned cells for CarmyA and 2D cultured cardiomyocytes. 

 

 

2. Key Marker Expressions

 

CarmyA expresses higher voltage-dependent K+ channel genes (HERG, KCNQ1, Kir1.2) than other commercially available iPS-derived cardiomyocytes. CarmyA also shows matured electrophysiological properties.

Fig.3. Relative comparison of gene expression levels for key cardiac markers. 

 

 

3. Drug Responses

 

CarmyA shows good arrhythmia responses (long QT, EAD and bradycardia) with introducing Terfenadine and Verapamil. 

Fig.4. Ca2+ imaging of CarmyA before and after exposure to Terfenadine and Verapamil. 

 

 

3.1. E4031

 

CarmyA shows clear intracellular Ca2+ wave prolongation (long QT) and EAD (Early After Depolarization) with dosing E4031, a typical HERG channel blocker.

Fig.5. Ca2+ fluorescence and motion result before and after CarmyA was exposed to E4031.

 

Fig.6. Dose response data when CarmyA was exposed to E4031.

 

 

3.2. Isoproterenol, Terfenadine

 

CarmyA shows more typical cardiotonic responses (compared with conventional iPS-derived cardiomyocytes) with showing tachycardia with being exposed to Isoproterenol – a typical cardiotonic drug. CarmyA also shows clearer Ca2+ wave prolongation with being exposed to Terfenadine – a typical long QT compounds.

Fig.7. Ca2+ imaging of CarmyA and conventional iPS-derived cardiomyocytes being exposed to Isoproterenol and Terfenadine. 

 

 

4. Mitochondria Activity

 

CarmyA shows stronger mitochondrial activities compared with conventional iPS-derived cardiomyocytes. 

Fig.8. Oxygen consumption rate (OCR) of CarmyA and conventional iPS-derived cardiomyocytes. 

 

 

5. Original Software for Fluorescence and Motion Analysis

 

Myoridge co-developed our original software being able to visualize both fluorescence and motion peaks. This software also provides good Ca2+ visualization in multi-well plates, therefore suitable good for high throughput analysis.

Fig.9. Outlook of fluorescence (left) and motion (right) analysis screen using the developed software.

 

Fig.10. Ca2+ visualization using 96 well plate.

 

 

6. Established Quality Control and Logistics Management

Myoridge established its own quality control systems with guaranteeing the cell numbers and key functions as cardiomyocytes. Customers are able to access our quality data sheet (certificates of analysis) upon request before placing orders. CarmyA are cryopreserved and delivered to world-wide using special cryogenic delivery services.

 

Specification of typical CarmyA cryopreserved cells.

Category Specification
Cell number  1.0 x 107
Viability after thawing  > 60%

Key Cardiac Expression

    αMHC expression

    HERG expression

    KCNQ1 expression

    Kir2.1 expression

 

Present

Present

Present

Present

Speciality Custom Projects

“We are flexible to produce bespoke cell products and services for our clients”.

 

 

1. Flexible formatting

  • Myoridge is able to produce iPS-derived cardiomyocytes with flexible formatting such as spheroids, sheet and even fiber.
  • Spheroids: Typically 100 to 1000 um diameter with using 3D platform and provided in 96 well plates.
  • Sheet: Suitable to observe pulsation. Flexible sheet sizes on demand.
  • Fiber: Suitable to observe pulsation and long-term culturing. Flexible fiber length on demand.

 

 

 

 

 

 

2. Disease Model Development

 

Myoridge is capable to produce disease modelled cardiomyocytes using integrated iPS-formatting, gene-editing and differentiation techniques. Please enquire for further details.

 

Products and Delivery

ID Product name Description Format
H-011107 CarmyA Cardiomyocytes derived from human iPSC 1.0 x 107 (2mL vial)
G-011107 CarmyA-GCaMP CarmyA expressing GCaMP guaranteed 1.0 x 107 (2mL vial)
M-011107  CarmyA-Monkey Cardiomyocytes derived from monkey iPSC 1.0 x 107 (2mL vial)
ME-01 CarmyA Maintenance media Maintenance Media (Myoridge) - for Human Cardiomyocytes 100 mL bottle
ME-02 CarmyA Thawing media Thawing Media (Myoridge) - for Human Cardiomyocytes 100 mL bottle
  • Please request for quote.
  • Cryopreserved cell vials are shipped using special dry-shipper with guaranteeing cryogenic temperature.
  • Thawing and plating media are separately shipped with dry-ice.
  • Shipping fee excluded.
  • Customers are responsible for local duty and taxes.
  • Standard terms and conditions are available upon request.

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